對于很多科(ke)研汪來說，細(xi)(xi)胞(bao)(bao)是(shi)(shi)脆(cui)弱又重要(yao)的(de)(de)(de)寶貝(bei)之一，承(cheng)擔著(zhu)重要(yao)的(de)(de)(de)實驗數據(ju)希望。尤(you)其是(shi)(shi)在(zai)凍存(cun)(cun)復(fu)蘇的(de)(de)(de)時(shi)候，經常會因為一些小細(xi)(xi)節導致問題。比如：沒有控(kong)制好細(xi)(xi)胞(bao)(bao)的(de)(de)(de)生長密度(du)細(xi)(xi)胞(bao)(bao)的(de)(de)(de)密度(du)對細(xi)(xi)胞(bao)(bao)的(de)(de)(de)生存(cun)(cun)質量(liang)有著(zhu)重要(yao)的(de)(de)(de)影(ying)響，畢竟它們是(shi)(shi)一群又不能擠著(zhu)了也不能孤獨的(de)(de)(de)嬌(jiao)貴寶寶。密度(du)過高(gao)會讓細(xi)(xi)胞(bao)(bao)沒有足夠的(de)(de)(de)生存(cun)(cun)空間，密度(du)過低會讓細(xi)(xi)胞(bao)(bao)無(wu)法互相(xiang)連接健康(kang)生長。建(jian)議大家在(zai)細(xi)(xi)胞(bao)(bao)接種前(qian)，一定(ding)要(yao)調整好適合細(xi)(xi)胞(bao)(bao)生長的(de)(de)(de)濃(nong)度(du)，提高(gao)細(xi)(xi)胞(bao)(bao)的(de)(de)(de)存(cun)(cun)活率。溫度(du)控(kong)制不達標慢凍快(kuai)融(rong)是(shi)(shi)細(xi)(xi)胞(bao)(bao)凍存(cun)(cun)復(fu)蘇的(de)(de)(de)*關鍵(jian)詞(ci)之一存(cun)(cun)細(xi)(xi)胞(bao)(bao)時(shi)需現(xian)配凍存(cun)(cun)液(ye)(如購買市面上通用(yong)的(de)(de)(de)含血(xue)清(qing)(qing)細(xi)(xi)胞(bao)(bao)凍存(cun)(cun)液(ye)。杭州長沙無(wu)血(xue)清(qing)(qing)細(xi)(xi)胞(bao)(bao)凍存(cun)(cun)液(ye)

無(wu)血(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)，含(han)多種保護(hu)(hu)(hu)劑(ji)(ji)成(cheng)分(fen)。一些保護(hu)(hu)(hu)劑(ji)(ji)，易于穿(chuan)透細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，避(bi)免細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)內部(bu)水(shui)分(fen)子形成(cheng)冰晶損傷細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，同時另一些保護(hu)(hu)(hu)劑(ji)(ji)不(bu)能(neng)穿(chuan)透細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，但可(ke)以在冰晶形成(cheng)之前，優先(xian)結合(he)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)外的水(shui)分(fen)子，降(jiang)低細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)外溶液(ye)(ye)的電解(jie)質濃度，減少陽離子進入細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的數量，為(wei)多種保護(hu)(hu)(hu)劑(ji)(ji)組合(he)，在細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)內外進行(xing)雙重保護(hu)(hu)(hu)，較大提(ti)高(gao)了(le)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)復蘇存(cun)(cun)活率(lv)。無(wu)血(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)不(bu)含(han)牛血(xue)清(qing)(qing)，無(wu)動物源組份。2-8℃即可(ke)穩定(ding)保存(cun)(cun)，無(wu)需冷(leng)凍(dong)，即取即用(yong)。凍(dong)存(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，無(wu)需程序降(jiang)溫。凍(dong)存(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)復蘇率(lv)在90%以上。重慶(qing)正規無(wu)血(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)廠家批發價無(wu)血(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)使用(yong)方法：加入適量的無(wu)血(xue)清(qing)(qing)型(xing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)(ye)于離心管中(zhong)。

無(wu)(wu)血清細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍存(cun)液(ye)的操(cao)(cao)作(zuo)規(gui)范：1、培養細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)至對數(shu)生長晚期，顯微鏡觀察(cha)其(qi)外觀、形態、有無(wu)(wu)污(wu)染(ran)等。選(xuan)擇(ze)狀態良好的細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)進(jin)行凍存(cun)操(cao)(cao)作(zuo)(注(zhu)：貼壁生長細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，用(yong)胰蛋白酶消化(hua)并用(yong)完全培養基終止消化(hua)，進(jin)行細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)計數(shu);懸(xuan)浮(fu)生長細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，進(jin)行細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)計數(shu))。2、500～1000g離(li)心(xin)5min，棄上清。3、加入適(shi)量(liang)的細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍存(cun)液(ye)，重(zhong)懸(xuan)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，使細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)濃(nong)度(du)達到1～10×106/ml，置(zhi)于(yu)凍存(cun)管中密閉。4、采取逐(zhu)級降(jiang)溫保存(cun)：4℃放置(zhi)20min，-20℃放置(zhi)30min，-70℃過夜，置(zhi)于(yu)液(ye)氮罐(guan)中長期存(cun)儲。注(zhu)意(yi)：務必超凈工(gong)作(zuo)臺內無(wu)(wu)菌操(cao)(cao)作(zuo)，避(bi)免污(wu)染(ran)。雜交瘤細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍存(cun)時(shi)應定(ding)期復蘇，以檢查(cha)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的活性和分泌抗(kang)體的穩定(ding)性。

無血(xue)清(qing)細(xi)胞(bao)(bao)(bao)凍存(cun)(cun)液通用于(yu)各(ge)種(zhong)動(dong)物(wu)細(xi)胞(bao)(bao)(bao)株(zhu)（tumour細(xi)胞(bao)(bao)(bao)和(he)常規細(xi)胞(bao)(bao)(bao)），大部分(fen)的(de)干細(xi)胞(bao)(bao)(bao)，凍存(cun)(cun)細(xi)胞(bao)(bao)(bao)可(ke)(ke)在-80℃長期(qi)(qi)保存(cun)(cun)。不(bu)含(han)動(dong)物(wu)源性蛋(dan)白，不(bu)含(han)血(xue)清(qing)成分(fen)，可(ke)(ke)有效減少各(ge)類(lei)細(xi)菌、病毒和(he)支原(yuan)體等(deng)(deng)污染，保證凍存(cun)(cun)細(xi)胞(bao)(bao)(bao)的(de)安全。該凍存(cun)(cun)液含(han)DMSO、葡(pu)萄糖等(deng)(deng)各(ge)種(zhong)細(xi)胞(bao)(bao)(bao)營養(yang)成分(fen)，提高細(xi)胞(bao)(bao)(bao)存(cun)(cun)活率和(he)活力(li)，亦適合于(yu)無血(xue)清(qing)培養(yang)細(xi)胞(bao)(bao)(bao)和(he)蛋(dan)白表達細(xi)胞(bao)(bao)(bao)。產品優勢：1、無需(xu)程(cheng)序性降(jiang)溫(wen)，可(ke)(ke)直接將(jiang)細(xi)胞(bao)(bao)(bao)置于(yu)-80℃凍存(cun)(cun)，凍存(cun)(cun)液無需(xu)稀釋。2、細(xi)胞(bao)(bao)(bao)可(ke)(ke)于(yu)-80℃長期(qi)(qi)保存(cun)(cun)。3、凍存(cun)(cun)液不(bu)含(han)血(xue)清(qing)等(deng)(deng)，較大降(jiang)低細(xi)胞(bao)(bao)(bao)污染的(de)可(ke)(ke)能(neng)性。保存(cun)(cun)方法：冰袋運輸，4℃保存(cun)(cun)，保質期(qi)(qi)一年。無血(xue)清(qing)細(xi)胞(bao)(bao)(bao)凍存(cun)(cun)液可(ke)(ke)用于(yu)其他類(lei)型(xing)哺(bu)乳動(dong)物(wu)細(xi)胞(bao)(bao)(bao)的(de)儲存(cun)(cun)。

無(wu)血清(qing)(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)使(shi)用(yong)(yong)方法及注(zhu)意事項(xiang)：無(wu)血清(qing)(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)：含多(duo)種(zhong)保護(hu)劑(ji)(ji)成分,一些(xie)保護(hu)劑(ji)(ji),易于(yu)穿透(tou)細(xi)(xi)(xi)胞(bao)(bao)(bao),避免(mian)細(xi)(xi)(xi)胞(bao)(bao)(bao)內(nei)部水分子(zi)形成冰晶損傷細(xi)(xi)(xi)胞(bao)(bao)(bao),同時另一些(xie)保護(hu)劑(ji)(ji)不(bu)能穿透(tou)細(xi)(xi)(xi)胞(bao)(bao)(bao),但可(ke)以(yi)在(zai)冰晶形成之(zhi)前,優先結(jie)合(he)細(xi)(xi)(xi)胞(bao)(bao)(bao)外的(de)(de)(de)(de)水分子(zi),降(jiang)低(di)細(xi)(xi)(xi)胞(bao)(bao)(bao)外溶液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)電解質(zhi)濃度(du),減少陽離(li)子(zi)進(jin)入細(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)數量,我公(gong)司無(wu)血清(qing)(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye),為多(duo)種(zhong)保護(hu)劑(ji)(ji)組(zu)合(he),在(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)內(nei)外進(jin)行(xing)雙重保護(hu),較大提高了細(xi)(xi)(xi)胞(bao)(bao)(bao)復蘇存(cun)(cun)(cun)(cun)活率(lv)。【產品用(yong)(yong)途】1.用(yong)(yong)于(yu)免(mian)疫細(xi)(xi)(xi)胞(bao)(bao)(bao)及干(gan)細(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)存(cun)(cun)(cun)(cun)儲(chu)。2.細(xi)(xi)(xi)胞(bao)(bao)(bao)保藏(zang)中(zhong)(zhong)(zhong)心，用(yong)(yong)于(yu)細(xi)(xi)(xi)胞(bao)(bao)(bao)株的(de)(de)(de)(de)長期(qi)保存(cun)(cun)(cun)(cun)。3.科研高校，細(xi)(xi)(xi)胞(bao)(bao)(bao)株凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。4.醫院樣本庫細(xi)(xi)(xi)胞(bao)(bao)(bao)樣本保存(cun)(cun)(cun)(cun)。【使(shi)用(yong)(yong)方法】1、細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)前準備（1）要(yao)(yao)求凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)的(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)在(zai)對數生(sheng)長期(qi)，且活率(lv)大于(yu)90%。（2）計(ji)算細(xi)(xi)(xi)胞(bao)(bao)(bao)密度(du)，一般要(yao)(yao)求密度(du)在(zai)1-3x10cells/mL，不(bu)同細(xi)(xi)(xi)胞(bao)(bao)(bao)系請參照附表。2、細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)過程(cheng)（1）將要(yao)(yao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)的(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)懸(xuan)液(ye)(ye)(ye)(ye)，進(jin)行(xing)細(xi)(xi)(xi)胞(bao)(bao)(bao)計(ji)數，計(ji)數后離(li)心，8003min即可(ke)。度(du)保存(cun)(cun)(cun)(cun)的(de)(de)(de)(de)無(wu)血清(qing)(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)緩慢(man)加入離(li)心后的(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)沉淀中(zhong)(zhong)(zhong)，根據密度(du)調整細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)用(yong)(yong)量。（3）反復吹(chui)吸混(hun)勻(yun)后，分裝(zhuang)于(yu)細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)管中(zhong)(zhong)(zhong)，每管500ul-1000ul，（建議500ul/管）。無(wu)血清(qing)(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)使(shi)用(yong)(yong)方法：將凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)管直接轉(zhuan)移至-80C水箱中(zhong)(zhong)(zhong)冷凍(dong)(dong)(dong)保存(cun)(cun)(cun)(cun)。廣州正規(gui)無(wu)血清(qing)(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)(ye)(ye)廠(chang)家直銷(xiao)

可以防(fang)止(zhi)或減少冷(leng)凍(dong)冰(bing)晶對細(xi)胞(bao)的(de)損傷作用。杭州長沙無血清細(xi)胞(bao)凍(dong)存液

無血清快速細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)液(ye)，通用(yong)于各(ge)(ge)(ge)種(zhong)動物(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)株。特別配(pei)方具有有效提高細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)活率和復(fu)蘇活力(li)。不含(han)動物(wu)來源性(xing)蛋白(bai)，能(neng)減(jian)少各(ge)(ge)(ge)類(lei)病毒(du)、霉菌(jun)和支(zhi)原體等的污(wu)染，確(que)保凍(dong)(dong)存(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)安(an)全(quan)。既(ji)適用(yong)于一般培養(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)的凍(dong)(dong)存(cun)，也適用(yong)于無血清培養(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)和蛋白(bai)表達細(xi)(xi)(xi)(xi)胞(bao)(bao)的凍(dong)(dong)存(cun)。產品(pin)特色：高安(an)全(quan)性(xing)，完全(quan)使(shi)(shi)用(yong)醫藥品(pin)等級(ji)原料進(jin)行生產，不含(han)動物(wu)成分，病毒(du)、霉菌(jun)和支(zhi)原體等污(wu)染可能(neng)性(xing)低，各(ge)(ge)(ge)批(pi)產品(pin)之間有更高的產品(pin)質量一致性(xing)。細(xi)(xi)(xi)(xi)胞(bao)(bao)存(cun)活率高，無批(pi)次差異。完全(quan)凍(dong)(dong)存(cun)液(ye)配(pei)方，可直接使(shi)(shi)用(yong)，方便簡捷，可直接存(cun)放(fang)于-70℃冰箱凍(dong)(dong)存(cun)，無需經過(guo)費時(shi)的程序降溫過(guo)程（省(sheng)時(shi)、省(sheng)力(li)、省(sheng)錢）。杭州長(chang)沙無血清細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)液(ye)

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